5-Ethynyl-cytidine (5-EC)

For general laboratory use.

Envio: shipped at ambient temperature

Condições de armazenamento: store at -20 °C
store dry and under inert gas
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C11H13N3O5

Peso molecular: 267.24 g/mol

CAS#: 65223-78-1

Pureza: ≥ 99 % (HPLC)

Forma: solid

Solubilidade: DMSO

Propriedades espectroscópicas: λmax 294 nm, ε 8.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
RNA synthesis monitoring^[1]

Descrição:
Ethynyl-labeled cytidine (5-EC) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EC is cell permeable and incorporates into nascent RNA instead of its natural analog cytidine.

The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1].

Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Referências selecionadas:
[1] Qu et al. (2013) 5-Ethynylcytidine as a new agent for detecting RNA synthesis in live cells by click chemistry. Anal. Biochem. 434:128.