λDNA/ HindIII Digest
ready-to-use
Catálogo Nº | Apresentação | Preço (R$) | Comprar |
---|---|---|---|
M-101S | 100 μg | Sob demanda | Adicionar ao Carrinho |
M-101L | 5 x 100 μg | Sob demanda | Adicionar ao Carrinho |
1 μg loaded onto 1.0 % agarose
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.
Validade: 12 months
Forma: liquid, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 8 % (w/v) Glycerol, Tartrazine and Xylene Cyanol FF
Concentração: 100 ng/μl
Descrição:
The HindIII digest of λ DNA yields the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125.
* The cohesive ends (12b cos site of bacteriophage lambda) of fragments 23130 bp and 4361 bp may be separated by heating to 65 °C for 5 min.
Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.
Recommended Load:
5 μl per lane
Preparation:
Lambda DNA (48.502 bp) was completely digested by HindIII, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.