Nucleotídeos Fluorescentes para Marcação de RNA
Table 1: Enyzmatic incorporation of fluorescent Nucleotides.
| cRNA Labeling by in vitro Transcription with... | 3'-RNA Labeling by… | |||||||
|---|---|---|---|---|---|---|---|---|
| Emission color | Nucleotide | Cat. No. | T7 RNA Polymerase | T3 RNA Polymerase | SP6 RNA Polymerase | yPAP | thT | T4 RNA Ligase |
| UTP | ||||||||
| green | Fluorescein-12-UTP | NU-821-FAMX | ✔1 | n/a | n/a | n/a | n/a | - |
| UTP-PEG5-AF488 | NU-821-PEG5-AF488 | ✔1 | n/a | n/a | n/a | n/a | - | |
| UTP-ATTO-488 | NU-821-488 | ✔1 | n/a | n/a | n/a | n/a | - | |
| yellow- green | UTP-ATTO-532 | NU-821-532 | ✔1 | n/a | n/a | n/a | n/a | - |
| yellow | UTP-Cy3 | NU-821-Cy3 | ✔2 | n/a | n/a | n/a | n/a | - |
| UTP-X-Cy3 | NU-821-X-CY3 | ✔2 | n/a | n/a | n/a | n/a | - | |
| UTP-ATTO-550 | NU-821-550 | ✔1 | n/a | n/a | n/a | n/a | - | |
| UTP-PEG5-AF555 | NU-821-PEG5-AF555 | ✔1 | n/a | n/a | n/a | n/a | - | |
| orange | UTP-PEG5-AF594 | NU-821-PEG5-AF594 | ✔1 | n/a | n/a | n/a | n/a | - |
| red | UTP-Cy5 | NU-821-Cy5 | ✔2 | n/a | n/a | n/a | n/a | - |
| UTP-X-Cy5 | NU-821-X-CY5 | n/a | n/a | n/a | n/a | n/a | - | |
| UTP-PEG5-AF647 | NU-821-PEG5-AF647 | ✔1 | n/a | n/a | n/a | n/a | - | |
| UTP-ATTO-647N | NU-821-647N | ✔1 | n/a | n/a | n/a | n/a | - | |
| near-IR | UTP-680 | NU-821-680 | ✔1,2 | n/a | n/a | n/a | n/a | - |
| CTP | ||||||||
| yellow | CTP-Cy3 | NU-831-Cy3 | ✔2 | n/a | n/a | n/a | n/a | - |
| red | CTP-Cy5 | NU-831-Cy5 | ✔2 | n/a | n/a | n/a | n/a | - |
| Cytidine-5’/3’-phosphate | ||||||||
| yellow | pCp-Cy3 | NU-1706-Cy5 | - | - | - | - | - | ✔2 |
| red | pCp-Cy5 | NU-1706-Cy5 | - | - | - | - | - | ✔2 |
yPAP: Yeast Poly A Polymerase
TdT: Terminal deoxynucleotidyl Transferase.
1: in-house tested
2: external reference (see datasheet)
n/a: no data available
- : not applicable