LEXSY BHI - Liquid Media Kit

For general laboratory use. Not intended for human or animal diagnostic or therapeutic uses.

Envio: Shipped at ambient temperature/on gel packs

Condições de armazenamento: Store components as indicated on individual labels

Validade: 12 months

Formulários:
For the growth of LEXSY hosts P10 (Cat.-No. LT-101) and T7-TR (Cat.-No. LT-110).

Descrição:
Three-component Kit for preparation of complex Leishmania cultivation medium.

Contente:
LEXSY BHI, liquid

Hemin stock solution, 500x (Cat.-No. ML-108):
0,25 % solution of Hemin in 30 % Triethanolamine, sterile

Pen-Strep stock solution, 200x (Cat.-No. ML-105):
10.000 U/ml of penicillin G sodium salt and 10.000 μg/ml of streptomycin sulfate in 0.85 % saline, sterile

Cat. No. || Content || Amount
ML-411S || 1 l medium2 ml Hemin5 ml Pen-Strep || for 1 l medium
ML-411L || 5 x 1 l medium10 ml Hemin25 ml Pen-Strep || for 5 l medium

Storage conditions:
LEXSY BHI, liquid:
Store at room temperature
Stable for 12 months

Hemin stock solution, 500x:
Store at 4 °C in the dark
Stable for 12 month

Pen-Strep stock solution, 200x:
Store at -20 °C
Stable for 12 month

Preparation of complete Leishmania cultivation medium:

Add to 1 l LEXSY BHI:
5.0 ml Pen-Strep solution
2.0 ml Hemin stock solution to a final concentration of 5 μg/ml

Final pH = 7.6 ± 0.2

Store in the dark at 4 °C.

Completed medium is useable for up to 2 weeks. If the completed medium is to be used after this period, appropriate amounts of Hemin and Pen-Strep have to be re-added.

Referências selecionadas:
[1] Zauner et al. (2018) Structural Analyses of Arabidopsis thaliana Legumain γ Reveal Differential Recognition and Processing of Proteolysis and Ligation Substrates. J Biol Chem 293: 8934