LEXSY host P10
for constitutive expression
|Catálogo Nº||Apresentação||Preço (R$)||Comprar / Observação|
|LT-101||3 x 1,6 ml||Sob demanda||Adicionar ao Carrinho|
For research use only! Not intended for human or animal diagnostic or therapeutic uses.
Envio: Shipped on dry ice
Condições de armazenamento: Store at -80 °C
Avoid freeze/thaw cycles
Upon arrival the glycerol stocks must be stored at -80 °C or inoculated into LEXSY BHI.
Validade: 12 months
The Leishmania tarentolae laboratory strain P10 is used as host strain for the constitutive LEXSY expression vectors.
3 vials with 1.6 ml each of frozen glycerol stocks of LEXSY host P10 (Leishmania tarentolae laboratory strain P10).
These stocks can be stored at -80 °C for at least 1 year. For reactivation see below.
Leishmania tarentolae, laboratory strain P10
1, Non-pathogenic for mammalians
Reactivation of LEXSY host:
Thaw glycerol stock on ice and inoculate the entire content of the vial into 10 ml of LEXSY BHI medium (Cat.-No. ML-411). Incubate at 26 °C and dilute as required.
Preparation of LEXSY BHI growth medium:
Dissolve 37 g/l LEXSY BHI powder (Cat.-No. ML-412) in deionized water and autoclave for 15 min at 121 °C. Add Hemin and PenStrep. Store at 4 °C and use within two weeks.
Preparation of glycerol stocks:
Add 1.2 ml of growing culture (ca. 6x107 cells/ml) to one vial with 0.4 ml 80 % glycerol, mix, incubate 10 min at RT, 1 h on ice and over night at -20 °C. Transfer to -80 °C. Strains can be stored this way for several years.
 Zauner et al. (2018) Structural Analyses of Arabidopsis thaliana Legumain γ Reveal Differential Recognition and Processing of Proteolysis and Ligation Substrates. J Biol Chem 293: 8934